Flow cytometric analysis of KARPAS-299 cells, untreated (blue) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min) (green), using Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (PE Conjugate). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
posted July 2009
revised June 2017
Protocol Id: 407
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (PE Conjugate) recognizes endogenous levels of IRAK4 protein only when phosphorylated at Thr345 and Ser346. This antibody does not react with singly phosphorylated proteins.
Mouse, Rat, Monkey, Bovine, Dog
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr345/Ser346 of human IRAK4 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb #11927.
Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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|12914S||100 µl (50 tests)||$ 327|