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Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of NDRG1 when phosphorylated at Thr346. This antibody likely cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr346 of NDRG1 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb #5482.

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

1.  Shimono, A. et al. (1999) Mech Dev 83, 39-52.

2.  Zhou, D. et al. (1998) Cancer Res 58, 2182-9.

3.  van Belzen, N. et al. (1997) Lab Invest 77, 85-92.

4.  Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.

5.  Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.

6.  Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.

7.  Stein, S. et al. (2004) J Biol Chem 279, 48930-40.

8.  Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.

9.  Nishio, S. et al. (2008) Cancer Lett 264, 36-43.

10.  Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.

11.  Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.

12.  Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.

13.  Murray, J.T. et al. (2004) Biochem J 384, 477-88.

Entrez-Gene Id 10397
Swiss-Prot Acc. Q92597

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate)