Render Target: STATIC
Render Timestamp: 2024-12-12T10:42:44.562Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:14:20.825
Product last modified at: 2024-11-08T12:30:11.637Z
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PDP - Template Name: Miscellaneous
PDP - Template ID: *******097325a

Protein A (HRP Conjugate) #12291

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  • WB

    Product Information

    Product Usage Information

    This product should be used in place of the secondary in the western blotting protocol.

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C.

    Protocol

    Product Description

    This Cell Signaling Technology product is useful for the detection of IgG. Conjugation of horseradish peroxidase (HRP) to protein A is obtained by cross-linking the amino groups on protein A with the carbohydrate groups on HRP.

    Specificity / Sensitivity


    Species Reactivity:

    All Species Expected

    Source / Purification

    Protein A (HRP Conjugate) is purified by gel filtration. The conjugate product is free from unconjugated protein A and HRP.

    Background

    Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and therefore can be used for detection and/or purification of antibodies. Due to its vast array of applications, protein A is the method of choice for many preparative and analytical immunological assays (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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