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Render Timestamp: 2024-11-12T10:06:12.876Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-05-10 06:29:18.751
Product last modified at: 2024-10-24T17:30:08.789Z
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Protein L (PE-Cy® 7 Conjugate) #31536

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    Product Information

    Product Usage Information

    Recommended Dilution: 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Product Description

    This Cell Signaling Technology® product is conjugated to phycoerythrin in combination with cyanine 7 (PE-Cy® 7) under optimal conditions.

    Specificity / Sensitivity

    Protein L (PE-Cy® 7 Conjugate) is recombinantly derived and genetically engineered such as to remove the cell wall-, cell membrane-, and albumin-binding regions of Protein L to enhance binding to immunoglobulins. Conjugation to PE-Cy® 7 makes this product ideally suited for direct flow cytometric analysis of cells expressing κ light chain-containing immunoglobulins as well as immunoglobulin fragments that contain κ light chains, such as scFvs.

    Species Reactivity:

    All Species Expected

    Background

    Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus (1). Unlike Protein A and Protein G, which differentially bind to the Fc region of immunoglobulin isotype heavy chains, Protein L contains Ig binding domains that interact with the κ light chain variable domain without disrupting paratope function (2-4). This attribute not only facilitates binding to a broader range of immunoglobulin classes and subclasses than either Protein A or Protein G but also allows for binding to Fab fragments and single chain variable fragments (scFvs) that contain κ light chain sequences. Protein L does, however, display specificity with respect to κ light chain subtypes. In human, Protein L binds to VκI, VκIII, and VκIV subtypes, whereas binding is restricted to VκI in mice (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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