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Product last modified at: 2025-02-05T09:00:10.949Z
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Streptavidin (PE Conjugate) #36777

    Product Information

    Product Description

    Streptavidin is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and is useful for detecting biotinylated proteins (1,2).

    Storage

    Store at 4°C. This product is stable for 12 months after receipt and is supplied in 100 mM sodium phosphate (pH 7.4), 100 mM NaCl, and 2 mM NaN3. If further dilution of the conjugate is required, use diluted material within one month.

    Protect from light. Avoid multiple freeze/thaw cycles.

    Specificity / Sensitivity

    Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

    Source / Purification

    Streptavidin is a protein that is derived from the bacteria Streptomyces avidinii. Streptavidin (PE Conjugate) is purified by SEC-HPLC.

    Background

    Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate and detect biotinylated proteins (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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