7-AAD Cell Staining Solution #47501
- F
Product Information
Product Usage Information
As a last step for flow cytometric assays, add 10 μl of 7-AAD Cell Staining Solution to 100 μl of sample. Incubate in the dark for 10 min at room temperature before analyzing cells on flow cytometer.
Storage
Fluorescent Properties
- Free dye excitation maximum = 503 nm
- Free dye emission maximum = 675 nm
- DNA complex excitation maximum = 543 nm
- DNA complex emission maximum = 655 nm
Product Description
Background
7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that binds poly(dG-dC) regions with an excitation maxima at 543 nm when bound to DNA (1). 7-AAD can be used as dead cell discriminating dye due to its exclusion from live cells with intact membranes (2).
Limited Uses
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