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47501
7-AAD Cell Staining Solution
Buffers & Dyes
Cellular Dyes

7-AAD Cell Staining Solution #47501

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Flow Cytometry Image 1: 7-AAD Cell Staining Solution
Flow cytometric analysis of Jurkat cells, untreated (left) or treated with Etoposide #2200 (25 μM, overnight; right), using 7-AAD Cell Staining Solution.

Product Usage Information

As a last step for flow cytometric assays, add 10 μl of 7-AAD Cell Staining Solution to 100 μl of sample. Incubate in the dark for 10 min at room temperature before analyzing cells on flow cytometer.

Storage

Store at 4ºC protected from light.

Fluorescent Properties

  • Free dye excitation maximum = 503 nm
  • Free dye emission maximum = 675 nm
  • DNA complex excitation maximum = 543 nm
  • DNA complex emission maximum = 655 nm

Product Description

7-AAD Cell Staining Solution is supplied in an aqueous buffered solution containing less than 0.09% sodium azide. It can be used to stain apoptotic cells.

Background

7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that binds poly(dG-dC) regions with an excitation maxima at 543 nm when bound to DNA (1). 7-AAD can be used as dead cell discriminating dye due to its exclusion from live cells with intact membranes (2).
  1. Schmid, I. et al. (1992) Cytometry 13, 204-8.
  2. Gill, J.E. et al. (1975) J Histochem Cytochem 23, 793-9.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 47501
Cat. # Size Qty. Price
47501S
1 ml $ 41

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