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Render Timestamp: 2024-11-04T10:36:13.954Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-10-11 13:16:10.183
Product last modified at: 2024-11-01T19:00:09.210Z
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PDP - Template Name: Cellular Dyes
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Alexa Fluor® 488 Phalloidin #8878

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    Product Information

    Product Usage Information

    To make a 800X stock, reconstitute the lyophilized material in 750 µl anhydrous DMSO. Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 488 Phalloidin can be diluted 1:800 (0.125 μl per 100 μl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.

    Note: Product usage information for this product has changed effective September 01 2024. Previous recommendations were to use either methanol or DMSO as a solvent for lyophilization of the material; It is now recommended to ONLY use DMSO.

    Storage

    This material is provided as lyophilized solid that is stable for 1 year at -20°C, desiccated and protected from light. Phalloidin conjugates should be reconstituted in anhydrous DMSO to make stock solutions, please refer to the directions for use for details. Once reconstituted in DMSO, stock solutions are stable for 1 year at -20°C. Stability in aqueous solutions is low and the conjugate should only be in the presence of an aqueous solution during incubation with cells.

    Product Description

    Alexa Fluor® 488 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is intended for use on fixed and permeabilized samples due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 6000 assays based on a 1:800 dilution and a 100 μl assay volume.

    Alexa Fluor® 488 Fluorescent Properties: Excitation: 495, Emission: 518.

    Background

    Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
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