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SignalStain® Aqueous Mounting Medium
Buffers & Dyes

SignalStain® Aqueous Mounting Medium #27290

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Product Usage Information

a. To prevent bubbles, do not shake the bottle. Carefully tip the bottle and dispense drops, using constant pressure on the bottle. If any bubbles are in the bottle tip, dispense and discard several drops before use.

b. Add 1 or 2 drops of SignalStain® Aqueous Mounting Medium on the stained section. When the coverslip is applied, the medium will spread evenly across the coverslipped area and will dry completely. Sections may be viewed immediately, but it will take approximately 10-15 minutes for the coverslip to become immobilized and several hours to be completely hardened.

c. If the view is obstructed with air bubbles or other artifacts, coverslips can be removed and sections can be remounted by soaking in buffer (PBS or TBST). Slides can be stored at room temperature for at least 12 months without the substrate fading.


Store at room temperature. SignalStain® Aqueous Mounting Medium is stable for 12 months. Keep container tightly sealed when not in use.

Product Description

SignalStain® Aqueous Mounting Medium is for use in immunohistochemical assays. This product is a hardsetting, aqueous-based mounting medium used to preserve tissue or cell preparations. SignalStain® Aqueous Mounting Medium can be used when the reaction products are soluble in alcohol or organic solvents. This product is compatible with most peroxidase and alkaline phosphatase enzymatic substrates.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
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To Purchase # 27290
Cat. # Size Qty. Price
60 ml