Immunofluorescent analysis of HeLa cells using MitoTracker® Green FM #9074 in normal culture media, with (left) or without (right) the addition of BackDrop® Green Background Suppressor.Learn more about how we get our images
BackDrop® Green Background Suppressor is a novel reagent designed to effectively suppress green background fluorescence and improve live cell image quality, eliminating the need for medium removal and potential cell loss. BackDrop® Green is supplied as a ready-to-use liquid in a convenient dropper bottle and is applied directly to live cell imaging samples.
To eliminate green background fluorescence and improve live cell image quality, apply 2 drops of BackDrop® Green Background Suppressor per 1 mL of media just prior to imaging. Cells should be imaged within 1 hour of application, however longer treatment has not been shown to harm cells.
Store at room temperature. This product is stable for 12 months.
Background signal from media components can decrease signal-to-noise ratio and result in reduced assay sensitivity. The degree of background suppression with BackDrop® Green can vary depending on the assay, but can often be significant. BackDrop® Green has been shown to improve results when used in conjugation with MitoTracker® Green FM #9074, LysoTracker® Green DND-26 #8783, and ER-Tracker™ Green (BODIPY® FL Glibenclamide) #8787, particularly if phenol red-free media is not used in place of normal media prior to imaging.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. BackDrop® is a registered trademark of Molecular Probes, Inc. BODIPY is a registered trademark of Life Technologies Corporation. LysoTracker® is a registered trademark of Molecular Probes, Inc. MitoTracker is a registered trademark of Molecular Probes, Inc.
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