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Cell Permeabilization Buffer (Triton™ X-100)
Buffers & Dyes

Cell Permeabilization Buffer (Triton X-100) #39487

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Product Usage Information

Centrifuge fixed cells and remove supernatant. Add Cell Permeabilization Buffer directly to fixed cells, using approximately 100 µl per 1 million cells. Incubate for 10 min at room temperature. Cells may be washed with phosphate-buffered saline (PBS) to remove Cell Permeabilization Buffer prior to antibody incubation, but it is not necessary. Note that the Triton X-100 detergent can reduce cell pellet adhesion, so care should be taken to retain cells when removing supernatant following centrifugation.


This product contains 0.05% sodium azide as a preservative and is stable for 12 months when stored at 4°C.

Product Description

This product is supplied as a 1X working solution for cell permeabilization in flow cytometry assays. Cell Signaling Technology recommends using this buffer prior to incubation with antibodies targeting intracellular proteins. Check the antibody product webpage to confirm that it is compatible with Triton X-100 permeabilization. This product contains enough material for 1,000 assays based on a 100 μl permeabilization volume.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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To Purchase # 39487
Cat. # Size Qty. Price
100 ml