Centrifuge fixed cells and remove supernatant. Add Cell Permeabilization Buffer directly to fixed cells, using approximately 100 µl per 1 million cells. Incubate for 10 min at room temperature. Cells may be washed with phosphate-buffered saline (PBS) to remove Cell Permeabilization Buffer prior to antibody incubation, but it is not necessary. Note that the Triton™ X-100 detergent can reduce cell pellet adhesion, so care should be taken to retain cells when removing supernatant following centrifugation.
This product contains 0.05% sodium azide as a preservative and is stable for 12 months when stored at 4°C.
This product is supplied as a 1X working solution for cell permeabilization in flow cytometry assays. Cell Signaling Technology recommends using this buffer prior to incubation with antibodies targeting intracellular proteins. Check the antibody product webpage to confirm that it is compatible with Triton™ X-100 permeabilization. This product contains enough material for 1,000 assays based on a 100 μl permeabilization volume.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Triton is a trademark of The Dow Chemical Company.