SignalStain® Citrate Unmasking Solution (10X) #14746
- IHC
Product Information
Product Usage Information
Dilute to a 1X unmasking solution using ddH2O just before use. Bring slides to a boil in 1X SignalStain® Citrate Unmasking Solution. Maintain at a sub-boiling temperature for 10 min. Cool slides on bench top for 30 min.
Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.
Storage
Product Description
Specificity / Sensitivity
Species Reactivity:
All Species Expected
Background
Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues antigens (1).
Limited Uses
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