SignalStain^® Citrate Unmasking Solution (10X) #14746

Product Usage Information

Dilute to a 1X unmasking solution using ddH₂O just before use. Bring slides to a boil in 1X SignalStain^® Citrate Unmasking Solution. Maintain at a sub-boiling temperature for 10 min. Cool slides on bench top for 30 min.

Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.

Storage:

Store 10X buffer at 4°C. At 10X, this buffer is stable for 12 months. Always prepare fresh 1X solutions daily.

Product Description

SignalStain^® Citrate Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if citrate is recommended for your specific product.

Background

Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues antigens (1).

1. Yamashita, S. (2007) Prog Histochem Cytochem 41, 141-200.