Render Target: STATIC
Render Timestamp: 2024-10-14T10:14:04.239Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:16:35.609
Product last modified at: 2024-10-04T11:30:09.421Z
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PDP - Template Name: Cellular Dyes
PDP - Template ID: *******0000253

DRAQ5® #4084

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    Product Information

    Product Usage Information

    Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:
    Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:500 (10 µM) in PBS or Antibody Dilution Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.
    Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

    Storage

    Store at 4°C protected from light.

    Background

    DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.