DyLight 554 Phalloidin #13054
- IF
Product Information
Product Usage Information
To make a 200X stock, reconstitute the lyophilized material in 1.5 ml pure methanol.
Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow Cell Signaling Technology protocol for immunofluorescence. Following incubation of the primary and secondary antibodies, DyLight 554 Phalloidin can be diluted 1:200 (0.5 µl per 100 µl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.
Storage
Product Description
DyLight 554 Phalloidin allows researchers to fluorescently label the cytoskeleton of fixed cells through the binding of phalloidin to F-actin. This product is not intended for use on live cells due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 3000 assays based on a 1:200 dilution and a 100 μl assay volume.
DyLight 554 Fluorescent Properties: Excitation: 551 nm, Emission: 572 nm.
Background
Actin is a ubiquitous eukaryotic protein and a major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells to control cell structure and motility (1). Actin exists mainly as a fibrous polymer called F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin resulting in an increase in the monomeric globular G-actin form (2). Phalloidin is a naturally occurring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, which rapidly binds to F-actin with strong affinity (3).
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.