Render Target: STATIC
Render Timestamp: 2024-11-08T10:05:53.228Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:14:34.600
Product last modified at: 2024-10-22T19:00:15.254Z
1% for the planet logo
PDP - Template Name: Cellular Dyes
PDP - Template ID: *******0000253

DyLight 650 Phalloidin #12956

Filter:
  • IF

    Product Information

    Product Usage Information

    To make a 200X stock, reconstitute the lyophilized material in 300 µl anhydrous DMSO.

    Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow Cell Signaling Technology protocol for immunofluorescence. Following incubation of the primary and secondary antibodies, DyLight 650 Phalloidin can be diluted 1:200 (0.5 µl per 100 µl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.

    Note: Product usage information for this product has changed effective September 01 2024. Previous recommendations were to use either methanol or DMSO as a solvent for lyophilization of the material; It is now recommended to ONLY use DMSO.

    Storage

    This material is provided as lyophilized solid that is stable for 1 year at -20°C, desiccated and protected from light. Phalloidin conjugates should be reconstituted in anhydrous DMSO to make stock solutions, please refer to the directions for use for details. Once reconstituted in DMSO, stock solutions are stable for 1 year at -20°C. Stability in aqueous solutions is low and the conjugate should only be in the presence of an aqueous solution during incubation with cells.

    Product Description

    DyLight 650 Phalloidin allows researchers to fluorescently label the cytoskeleton of fixed cells through the binding of phalloidin to F-actin. This product is not intended for use on live cells due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 600 assays based on a 1:200 dilution and a 100 μl assay volume.

    DyLight 650 Fluorescent Properties: Excitation: 651 nm, Emission: 672 nm.

    Background

    Actin is a ubiquitous eukaryotic protein and a major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells to control cell structure and motility (1). Actin exists mainly as a fibrous polymer called F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin resulting in an increase in the monomeric globular G-actin form (2). Phalloidin is a naturally occurring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, which rapidly binds to F-actin with strong affinity (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.