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SignalStain® EDTA Unmasking Solution (10X)
Buffers & Dyes

SignalStain® EDTA Unmasking Solution (10X) #14747

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  1. IHC
Immunohistochemistry Image 1 - SignalStain® EDTA Unmasking Solution (10X)

Product Usage Information

Dilute to a 1X Unmasking Solution using ddH2O just before use. Bring slides to a boil in 1X SignalStain® EDTA Unmasking Solution. Follow with 15 min at a sub-boiling temperature. No cooling is necessary.

Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.


Store 10X buffer at 4°C. At 10X, this buffer is stable for 12 months. Always prepare fresh 1X solutions daily.

Product Description

SignalStain® EDTA Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if this solution is recommended for your specific product.


Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues or antigens (1).

  1. Yamashita, S. (2007) Prog Histochem Cytochem 41, 141-200.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.

To Purchase # 14747S
Product # Size Price
25 ml $ 20
125 ml $ 84