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8787
ER-Tracker™ Green (BODIPY® FL Glibenclamide)
Buffers & Dyes
Cellular Dyes

ER-Tracker™ Green (BODIPY® FL Glibenclamide) #8787

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Immunofluorescence Image 1: ER-Tracker™ Green (BODIPY® FL Glibenclamide)

Confocal immunofluorescent analysis of live HeLa cells using ER-Tracker™ Green (BODIPY® FL Glibenclamide) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Product Usage Information

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX!

To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.

Storage

Store lyophilized solid or solution at –20°C dessicated and protected from light. Once reconstituted in DMSO, the solution should be aliquoted to avoid freeze-thaw cycles. If stored properly, this reagent is stable for 6 months.

Product Description

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight: 783.10 g/mol

Background

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY® FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
BackDrop® is a registered trademark of Molecular Probes, Inc.
BODIPY is a registered trademark of Life Technologies Corporation.
DRAQ5 is a registered trademark of Biostatus Limited.
To Purchase # 8787S
Product # Size Price
8787S
100 µg $ 323