Store at 4ºC. All components in this kit are stable for at least 6 months when stored at 4ºC.
NOTE: Precipitation may occur in some kit components. The presence of precipitate does not affect the performance of the reagent.
Additional Reagents (Not Supplied):
NOTE: Fixable viability dyes such as Ghost Dye(TM) Violet 510 Viability Dye #59863 should be added prior to fixation, following the dye product recommended protocol. Proceed with fixation once excess dye has been removed.
NOTE: Antibodies targeting CD markers or other extracellular epitopes may be added prior to fixation. The antibodies will remain bound to the target of interest during the fixation process. A wash step prior to fixation may be performed but is not necessary.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
revised June 2020
Protocol Id: 1744
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