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XML generation date: 2024-09-30 11:31:06.080
Product last modified at: 2024-10-01T07:00:57.755Z
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Human Fc Receptor Blocking Solution #58948

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    Product Information

    Product Usage Information

    Prior to immunostaining, treat cells with Human Fc Receptor Blocking Solution for 10 min at room temperature, using 2.5 μg/1 million cells in approximately 100 μL Antibody Dilution Buffer (may be purchased as ready-to-use Flow Cytometry Antibody Dilution Buffer #13616 or prepared as a solution of 0.5% Bovine Serum Albumin in 1X Phosphate Buffered Saline). No wash step is needed before incubating cells with primary antibodies.

    Storage

    Supplied in PBS solution (pH 7.2). Stable at 4°C for 1 year after receipt. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    Human Fc receptors cross-react with certain subclasses of IgG from species such as mouse, rabbit, and rat. When cells of interest express high levels of Fc receptor protein (for example, macrophage/monocyte lineages), pre-incubating live cells with Human Fc Receptor Blocking Solution prior to immunostaining will reduce this cross-reactivity.

    Specificity / Sensitivity


    Species Reactivity:

    Human

    Source / Purification

    Human Fc Receptor Blocking Solution was expressed in human 293 cells.

    Background

    Human Fc Receptor Blocking Solution contains a modified form of human IgG1 that blocks human Fc receptors.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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