Thaw 10X PTMScan® IAP buffer at room temperature or at 37 ºC. Ensure all contents are dissolved as some components may have precipitated upon freezing. If necessary, dissolve by shaking gently. Before use, dilute with Milli-Q or equivalenly purified H2O to 1X buffer (e.g., pipet 0.5 ml 10X IAP buffer from the vial into 4.5 ml H2O).
1X Buffer Components:
50 mM MOPS/NaOH pH 7.2
10 mM Na2HPO4
50 mM NaCl
Supplied as a 10X solution. Store at -20°C. Prepared 1X IAP buffer can be stored up to one month at 4°C.
PTMScan® IAP Buffer is used to reconstitute lyophilized peptides prior to immunoaffinity purification (IAP).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.