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11932
Image-iT® FX Signal Enhancer
Buffers & Dyes
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Image-iT® FX Signal Enhancer #11932

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Immunofluorescence Image 1: Image-iT® FX Signal Enhancer

Confocal immunofluorescent analysis of MCF7 cells using EpCAM (D1B3) Rabbit mAb #2626 (green) either untreated (left), or treated with Image-iT® FX Signal Enhancer (right). Note the reduction in non-specific, nucleolar background following pre-treatment with Image-iT® FX Signal Enhancer.

Product Usage Information

Following specimen preparation:

1. Permeabilize the cells in Permeabilization Buffer for 5 minutes at room temperature.

2. Rinse three times in PBS for 5 minutes each.

3. Apply 3–4 drops of Image-iT™ FX Signal Enhancer (or sufficient volume to cover the cells) and incubate for 30 minutes at room temperature.

4. Rinse three times with PBS for 5 minutes each.

5. Block specimen in Blocking Buffer for 60 minutes.

6. While blocking, prepare primary antibody by diluting as indicated on datasheet in Antibody Dilution Buffer.

7. Aspirate blocking solution, apply diluted primary antibody.

8. Incubate overnight at 4°C.

9. Rinse three times in PBS for 5 minutes each.

10. Coverslip slides with Prolong® Gold Antifade Reagent.

11. For best results, examine specimens immediately using appropriate excitation wavelength. For long-term storage, store slides flat at 4°C protected from light.

Storage

Store at room temperature. This product is stable for 12 months.

Product Description

Alexa Fluor® and many other anionic fluorescent dyes and proteins can bind nonspecifically with cationic cell and tissue constituents. By efficiently blocking these nonspecific electrostatic interactions, Image-iT® FX Signal Enhancer can dramatically improve the signal-to-noise ratio of immunolabeled cells and tissues. Image-iT® is a liquid that is applied directly to slides or coverslips containing fixed and permeabilized cell or tissue samples prior to staining with fluorescent probes.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 11932S
Product # Size Price
11932S
10 ml $ 117