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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Reagents Not Supplied

  1. Unconjugated or conjugated primary antibody.
  2. Fluorochrome-conjugated secondary antibody (if applicable).
NOTE: When using any primary or fluorochrome-conjugated secondary antibody for the first time, titrate the antibody to determine which dilution allows for the strongest specific signal with the least background for your sample.
  1. Methanol (if necessary).
  2. Antifade mounting reagent, such as ProLong® Gold Antifade Reagent (#9071), with DAPI (#8961).

Product Description

The Immunofluorescence Application Solutions Kit is designed to conveniently provide the major supporting reagents needed for immunofluorescence in cell cultures (IF-IC) or frozen samples (IF-F). The reagents in this kit are thoroughly validated using our IF-approved antibodies and will perform optimally with the kit’s recommended protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents for 100 assays based on a 100 µl assay volume.

IMPORTANT: Please refer to the antibody data sheet to determine if it is validated and approved for use on cultured cell lines (IF-IC) or frozen tissues sections (IF-F) and for information regarding appropriate antibody dilution.

Some primary antibodies may require methanol fixation or permeabilzation, which will be noted on the data sheet. Methanol is not included in this kit.

Product Usage Information

Storage: All components in this kit are stable for at least 12 months when stored at the recommended temperature and left unused. Formaldehyde fixative should be used within one month once opened.Upon receipt, #12528 and #12606 should be stored at room temperature. #12411 and #12378 should be stored at 4°C.

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
ProLong is a registered trademark of Life Technologies Corporation.
Triton is a trademark of The Dow Chemical Company.

Immunofluorescence Application Solutions Kit