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Commit: 7ed46ecc04b401f23a28df741b5078df405d23e4
XML generation date: 2026-05-15 13:24:41.052
Product last modified at: 2026-04-10T07:00:53.923Z
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PDP - Template Name: Buffer
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Immunofluorescence Blocking Buffer #12411

    Product Information

    Storage

    This product contains 0.05% sodium azide as a preservative and is stable for 12 months when stored at 4°C. Peroxidase-based fluorescence assays may be negatively affected by sodium azide (1).

    Product Description

    This blocking reagent is designed to reduce noise originating from nonspecific protein-protein interactions in immunofluorescence assays. The core formulation includes goat serum as a protein blocker mixed with a mild detergent to facilitate permeabilization of cellular membranes.

    Cell Signaling Technology recommends using the buffer in accordance with our protocols for cultured cells (IF-IC) and frozen tissue sections (IF-F) to ensure accurate and reproducible results.

    The product is supplied as a 1X working solution and contains enough material for 500 assays based on a 100 μl assay volume.

    Background

    Alternate Names

    companion; complementary; IF; Immunofluorescence; reagent

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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