This product contains 0.05% sodium azide as a preservative and is stable for 12 months when stored at 4°C. Peroxidase-based fluorescence assays may be negatively affected by sodium azide (1).
This blocking reagent is designed to reduce noise originating from nonspecific protein-protein interactions in immunofluorescence assays. The core formulation includes goat serum as a protein blocker mixed with a mild detergent to facilitate permeabilization of cellular membranes.
Cell Signaling Technology recommends using the buffer in accordance with our protocols for cultured cells (IF-IC) and frozen tissue sections (IF-F) to ensure accurate and reproducible results.
The product is supplied as a 1X working solution and contains enough material for 500 assays based on a 100 μl assay volume.