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Lambda Protein Phosphatase Kit
Buffers & Dyes
Buffer Kit

Lambda Protein Phosphatase Kit #89726

Citations (0)
Western blot analysis of extracts from serum-starved MCF7 cells, untreated (-) or treated (+) with combinations of the following treatments as indicated: human IGF-1 (hIGF-1; 100 ng/mL, 10 min) and lambda protein phosphatase, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).
To Purchase # 89726
Cat. # Size Qty. Price
1 Kit

Product Includes Quantity (with Count) Storage Temp
Lambda Protein Phosphatase 1 x 250 µl -80°C
Buffer for Protein MetalloPhosphatases (PMP) 1 x 1 ml -20°C
MnCl2 for PMP 1 x 1 ml -20°C

Product Usage Information

1. Add protein sample and H2O (if necessary) to make a total volume of 40 μL.

2. Add 5 μL of 10X Buffer for Protein MetalloPhosphatases (PMP) and 5 μL of 10 mM MnCl2 for PMP to make a total reaction volume of 50 μL.

3. Add 1 μL of Lambda Protein Phosphatase.

4. Incubate at 30°C for 30 min.

Note: The optimal incubation times and enzyme concentrations must be determined empirically for each individual substrate.


All components in this kit are stable for 24 months when stored at the recommended temperature. Aliquot to avoid repeated freeze/thaw cycles.

Product Description

The Lambda Protein Phosphatase Kit provides reagents that, when combined, have activity toward phosphorylated serine, threonine, and tyrosine residues. Lambda Protein Phosphatase is the 221 amino acid product of the ORF221 open reading frame on bacteriophage lambda. It is also an Mn2+ dependent protein phosphatase that has the ability to release phosphate groups from phosphorylated serine, threonine, and tyrosine residues in proteins. Lambda Protein Phosphatase has a concentration of 400,000 units per mL.
Activity 800,000 units per mg

Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 min at 30°C in a total reaction volume of 50 µL.

Specificity / Sensitivity

The Lambda Protein Phosphatase Kit is active toward phosphorylated serine, threonine, and tyrosine residues.

Source / Purification

The Lambda Protein Phosphatase component is produced in a strain of E. coli that carries the bacteriophage lambda ORF221 open reading frame. The product is isolated using the T7 expression system.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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