Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX!
To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.
Store at -20°C dessicated and protected from light. This reagent is stable for 12 months. Seal vial tightly after use to refreeze. Avoid excessive freeze-thaw cycles.
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|8783S||500 µl (10 x 50 ul)||