Confocal immunofluorescent analysis of live HeLa cells, untreated (left) or chloroquine-treated (50 μM, 4 hr; right), using LysoTracker® Green DND-26 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX!
To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.
Store at -20°C dessicated and protected from light. This reagent is stable for 12 months. Seal vial tightly after use to refreeze. Avoid excessive freeze-thaw cycles.
LysoTracker® Green DND-26 is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight 398.69 g/mol
LysoTracker® Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
BackDrop® is a registered trademark of Molecular Probes, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
LysoTracker® is a registered trademark of Molecular Probes, Inc.