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8783
LysoTracker® Green DND-26
Buffers & Dyes
Cellular Dyes

LysoTracker® Green DND-26 #8783

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  1. IF
Immunofluorescence Image 1: LysoTracker® Green DND-26

Confocal immunofluorescent analysis of live HeLa cells, untreated (left) or chloroquine-treated (50 μM, 4 hr; right), using LysoTracker® Green DND-26 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Product Usage Information

Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX!

To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.

Storage

Store at -20°C dessicated and protected from light. This reagent is stable for 12 months. Seal vial tightly after use to refreeze. Avoid excessive freeze-thaw cycles.

Product Description

LysoTracker® Green DND-26 is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight 398.69 g/mol

Background

LysoTracker® Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
BackDrop® is a registered trademark of Molecular Probes, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
LysoTracker® is a registered trademark of Molecular Probes, Inc.
To Purchase # 8783S
Product # Size Price
8783S
500 µl  (10 x 50 ul) $ 214