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8553
PMSF
Buffers & Dyes
Buffer

PMSF #8553

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WB Image 1 - PMSF

Chemical Structure of PMSF

Product Usage Information

For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection. Store lyophilized at RT for 24 months protected from direct light. Once in solution, PMSF can be stored at -20ºC for up to 3 months, protected from light.

Storage

Store at Room Temperature

Product Description

Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806). Molecular Formula: C7H7FO2S Molecular Weight: 174.19 CAS: 329-98-6

Molecular Formula

C7H7FO2S

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 8553S
Product # Size Price
8553S
34.84 mg $ 21