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4087
Propidium Iodide (PI)/RNase Staining Solution
Buffers & Dyes
Cellular Dyes

Propidium Iodide (PI)/RNase Staining Solution #4087

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Citations (6)
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  1. IF
  2. F
Immunofluorescence Image 1: Propidium Iodide (PI)/RNase Staining Solution

Confocal immunofluorescent analysis of rat cerebellum using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (blue pseudocolor) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.

Immunofluorescence Image 1: Propidium Iodide (PI)/RNase Staining Solution

Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb #3873 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.

Flow Cytometry Image 1: Propidium Iodide (PI)/RNase Staining Solution

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate) #9708 and Propidium Iodide (PI)/RNase Staining Solution (DNA content).

Product Usage Information

As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis.

Formulation

Supplied in aqueous buffered solution containing less than 0.09% sodium azide. As with all intercalating DNA dyes, take all necessary safety precautions when using.

Storage

Store at 4°C protected from light, do not aliquot or dilute. The solution is stable for 12 months.Concentration:PI = 10ug/mlRNase = 300 ug/ml

Fluorescent Properties

  • Ex(Max):535 nm
  • Em(Max): 617 nm

Background

Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. (1-3)

  1. Suzuki, T. et al. (1997) J Histochem Cytochem 45, 49-53.
  2. Jones, K.H. and Kniss, D.A. (1987) J Histochem Cytochem 35, 123-5.
  3. Deitch, A.D. et al. (1982) J Histochem Cytochem 30, 967-72.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
To Purchase # 4087S
Product # Size Price
4087S
100 ml $ 128