View Featured Offers >>
SignalStain® Radiant Yellow Peroxidase Substrate Kit
Buffers & Dyes
Detection System Kit

SignalStain® Radiant Yellow Peroxidase Substrate Kit #69644

Citations (0)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Vimentin (D21H3) XP® Rabbit mAb #5741.

Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 and SignalStain® Radiant Yellow Peroxidase Substrate Kit.
To Purchase # 69644
Cat. # Size Qty. Price
1 Kit  (1100 slides)

Product Includes Quantity
SignalStain® Radiant Yellow Diluent 110 ml
SignalStain® Radiant Yellow Chromogen Concentrate 3 ml

Product Usage Information

1. To prepare the Radiant Yellow/HRP chromogen-substrate working solution, add 1 drop SignalStain® Radiant Yellow Chromogen Concentrate to 1 mL SignalStain® Radiant Yellow Diluent. Mix well before use. Working solutions are stable for up to 1 day when stored at 4ºC. For optimal results, prepare fresh reagent.

2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 8-20 minutes generally provides an acceptable staining intensity.

3. Wash slides for 5 minutes in water.

4. Counterstain, if desired. Radiant Yellow is compatible with Hematoxylin #14166.

5. After rinsing, slides should be air dried and mounted using SignalStain® Aqueous Mounting Medium #27290. Radiant Yellow is not compatible with alcohol.

Alternatively, for best color preservation and long-term storage, SignalStain® Mounting Prep Solution #50815 can be used after counterstaining to enable mounting with non-aqueous mounting medium, SignalStain® Mounting Medium #14177.

Appropriate personal protective equipment should be worn when handling Radiant Yellow. Dispose of in accordance with local regulations.


Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 6 months upon receipt if the reagents are not combined.

Product Description

The SignalStain® Radiant Yellow Peroxidase Substrate Kit contains all of the necessary reagents to prepare a working solution of Radiant Yellow for staining tissue sections. The Radiant Yellow working solution is compatible with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, HRP, Mouse #8125, HRP, Rat #72838, and HRP, Goat #63707), yielding a yellow reaction product that can provide color contrast in pigmented tissues such as melanoma.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.