Product Information
1. Following gel electrophoresis, rinse gel briefly with diH2O to remove any detergent that may inhibit staining.
2. Invert bottle of TurboBlue Protein Gel Stain several times prior to use.
3. Incubate gel with gentle rocking in 25 mL of TurboBlue Protein Gel Stain for 60 min while covered at room temperature. Incubation may be extended overnight without overstaining.
Note: The staining solution volume is dependent on container size.
a. For best results, add enough TurboBlue Protein Gel Stain to cover the gel fully.
4. Optional: Wash gel in diH2O 3 times for 5 min at room temperature, or until desired destaining has occurred. Otherwise, rinse gel briefly with diH2O before imaging.
5. Proceed with imaging by photographing in visible light, imaging with white light, or by near-IR fluorescence gel scanner.
6. Disposal: TurboBlue Protein Gel Stain is a non-hazardous, non-toxic, 100% aqueous solution that can be disposed of down the drain once neutralized. The staining solution is acidic and must be neutralized before disposing down the drain. This can be done by adding 743 uL 1N sodium hydroxide per 1 mL of TurboBlue Protein Gel Stain and mixing well. This can also be done by adding 30 mg sodium hydroxide pellets per 1 mL of TurboBlue Protein Gel Stain and stirring to dissolve completely.
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| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 24053S | 1 liter |
|
