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SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit
Buffers & Dyes
Detection System Kit

SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit #12824

Citations (0)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Vimentin (D21H3) XP® Rabbit mAb #5741.

Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653 and SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit.
To Purchase # 12824
Cat. # Size Qty. Price
1 Kit  (1100 slides)

Product Includes Quantity
SignalStain® Ultra Blue Diluent 110 ml
SignalStain® Ultra Blue Chromogen Concentrate 3 ml

Product Usage Information

1. To prepare the Ultra Blue/AP chromogen-substrate working solution, add 1 drop SignalStain® Ultra Blue Chromogen Concentrate to 1 mL SignalStain® Ultra Blue Diluent. Mix well before use. Working solutions are stable for up to 1 day when stored at 4ºC. For optimal results, prepare fresh reagent.

2. After incubation with SignalStain® Boost IHC Detection Reagent or other AP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 8-20 minutes generally provides an acceptable staining intensity.

3. Wash slides for 5 minutes in water.

4. Counterstain, if desired. Note that Hematoxylin #14166 is unlikely to provide sufficient contrast with Ultra Blue.

5. Mount coverslips. After rinsing off the counterstain, air dry the slides for 20 minutes or longer. Permanently mount coverslips with SignalStain® Aqueous Mounting Medium #27290. Alternatively, slides may be dehydrated through graded alcohols and cleared in a xylene substitute, then mounted with a permanent mounting medium, SignalStain® Mounting Medium #14177. Ultra Blue is not compatible with xylene.

For best color preservation and long-term slide storage, it is recommended to use SignalStain® Mounting Prep Solution #50815 after counterstaining.

Appropriate personal protective equipment should be worn when handling Ultra Blue. Dispose of in accordance with local regulations.


Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 6 months upon receipt if the reagents are not combined.

Product Description

The SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit contains all of the necessary reagents to prepare a working solution of Ultra Blue for staining tissue sections. The Ultra Blue working solution is compatible with alkaline phosphatase (AP) detection systems such as the SignalStain® Boost IHC Detection Reagents (AP, Rabbit #18653, AP, Mouse #31926, AP, Rat #15764, and AP, Goat #26927), yielding a heat stable, blue reaction product that is also fluorescent.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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