CAR (Whitlow/218 Linker) Cell Enrichment Kit #65686
Product Information
Protocol
Product Description
Bead Specifications:
Releasable Streptavidin Beads are paramagnetic polystyrene beads (4.5 μm diameter) coupled to streptavidin via a DNA linker susceptible to cleavage by DNase I. The beads provided in this kit are not recommended for the isolation of phagocytic cells as the beads are likely to be engulfed by these cells.
Specificity / Sensitivity
Biotinylated Whitlow/218 Linker (E3U7Q) Rabbit mAb recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker.
Species Reactivity:
All Species Expected
Background
Magnetic bead-based immunoaffinity cell enrichment is a gentle purification method that can be leveraged, in part, to facilitate a robust interrogation of the biology of immune cells that are engineered to express CARs. For example, isolation of rare subsets of CAR positive cells from a complex, heterogeneous population of cells presents the opportunity for extensive downstream analyses using single-cell omics assays. In the context of CAR cell engineering, magnetic bead-based immunoaffinity cell enrichment can also be useful in scenarios when delivery of the CAR transgene is inefficient, resulting in a small population of cells expressing the CAR transgene.
Limited Uses
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