NOTE: Precipitation may occur when reagents are stored at −20°C. Warm reagents to 37°C if necessary to dissolve precipitate.
NOTE: Cell lysate plate can be stored at −80°C for future use.
NOTE: We recommend reading the plate immediately and recording RFU reading at time 0 hr. This will help determine if there is significant change in RFU at the end of incubation.
NOTE: This protocol has been tested in 384-well plate format, please adjust the volume proportionally based on the plate capacity. For example, if using 384-low volume plate, use 20 µl substrate solution B and 2.5 µl lysate.
NOTE: We recommend reading plates after 1 hr incubation. If the signal is too weak, increase incubation period to observe significant change in signal strength. If significant increase is signal strength is not observed, more lysate may be necessary.
posted October 2012
Protocol Id: 74
|CAS||67-68-5, 26093-31-2, 9002-93-1, 7681-49-4, 13472-36-1, 56-81-5, 3483-12-3,|
Explore pathways + proteins related to this product.
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.