NOTE: Precipitation may occur when reagents are stored at −20°C. Warm reagents to 37°C if necessary to dissolve precipitate.
NOTE: Cell lysate plate can be stored at −80°C for future use.
NOTE: We recommend reading the plate immediately and recording RFU reading at time 0 hr. This will help determine if there is significant change in RFU at the end of incubation.
NOTE: This protocol has been tested in 384-well plate format, please adjust the volume proportionally based on the plate capacity. For example, if using 384-low volume plate, use 20 µl substrate solution B and 2.5 µl lysate.
NOTE: We recommend reading plates after 1 hr incubation. If the signal is too weak, increase incubation period to observe significant change in signal strength. If significant increase is signal strength is not observed, more lysate may be necessary.
posted October 2012
Protocol Id: 74
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