|Product Includes||Quantity (with Count)|
|Cell Proliferation Tracer Dye, Violet 450||1 x 1 ea|
|Anhydrous DMSO||1 x 150 µl|
NOTE: The following protocol is a general labeling procedure. Because of differences in cell types and variations in culture conditions, optimization of the dye concentration, staining time, and/or staining temperature may be necessary. Higher dye concentrations may be required to track more cell generations, while lower concentrations may be sufficient to track fewer divisions. We recommend using the lowest dye concentration that yields sufficient signal for your assay, because cell proliferation dyes can be toxic to cells at high concentrations.
NOTE: Cell Proliferation Tracer dyes are susceptible to hydrolysis. Therefore, the DMSO stock solution should only be prepared on the day of use, and not subjected to freeze/thaw cycles. The dyes should only be added to aqueous buffer immediately before staining. Do not use buffers containing Tris or other free amines.
Additional Reagents (Not Supplied):
Note: Staining can be performed in cell culture medium containing serum, however, this results in 5-10 fold lower fluorescent signal compared to labeling in buffer without serum or other proteins.
posted January 2020
Protocol Id: 1950
Due to their inherent metabolic stability once inside a cell, fluorescent proliferation dyes partition in an equal manner between daughter cells during the M phase of the cell cycle. This allows the principle of dye dilution to be leveraged as a means to trace multiple rounds of cell proliferation using flow cytometry. Added benefits of proliferation dyes are that they are non-radioactive and do not require cells to be actively synthesizing DNA for efficient uptake (1-3).
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