CellSimple™ Mitochondrial Membrane Potential Assay Kit (I) is a fluorescent assay designed for use with the CellSimple™ Cell Analyzer. It detects the mitochondrial membrane potential in living cells. The kit includes the cationic dye JC-1 and a mitochondrial membrane potential disruptor CCCP (carbonyl cyanide 3-chlorophenylhydrazone). JC-1 is a cell membrane permeable, fluorescent dye with green emission (~520 nm). When JC-1 accumulates in intact mitochondria, the dye forms aggregates that lead to orange-red fluorescence (~590 nm). The mean fluorescence intensity (MFI) of the orange-red emission can be used as an indicator for mitochondrial membrane potential.
All Species Expected
The CellSimple™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.
Mitochondria function as the main cellular powerhouse and play important roles in other processes, such as steroid metabolism, calcium homeostasis, apoptosis, and cellular proliferation. Mitochondrial membrane potential is a key indicator of mitochondrial function and cell health (1,2). The dissipation of mitochondrial membrane potential is considered an early indicator of apoptosis (3).
JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide) is a cell membrane permeable, cationic dye. In normal cells, JC-1 concentrates in mitochondria to form aggregates in response to high membrane potential. Decreased mitochondrial membrane potential results in dispersal of mostly monomeric JC-1 throughout the cell. When excited at 490 nm, JC-1 monomers emit a green fluorescence with a maximum at ~520 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. Therefore, the fluorescence intensity of the orange-red emission and the ratio of orange-red fluorescence to green fluorescence can be used to measure mitochondrial membrane potential and serve as an indicator of overall cell health (4).
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.