|Product Includes||Quantity (with Count)||Solution Color|
|cAMP Rabbit mAb Coated Microwells||1 x 96 tests|
|cAMP-HRP Conjugate||1 x 11 ml||Red|
|cAMP Standard (2.4 uM)||1 x 1 ml|
|TMB Substrate 7004||1 x 11 ml|
|STOP Solution 7002||1 x 11 ml|
|Sealing Tape||1 x 2 ea|
|ELISA Wash Buffer (20X) 9801||1 x 10 ml|
|Cell Lysis Buffer (10X) 9803||1 x 15 ml|
NOTE: This procedure is for a 96-well tissue culture plate. It can be modified for other tissue culture plates (6, 12, 24, or 48 well).
NOTE: If cell debris is observed it can be removed by brief centrifugation of the plate and transfer of the clear lysates to a new 96 well plate.
NOTE: Watch the color as it being developed since it may be necessary to stop the reaction before 30 minutes.
NOTE: To determine the absolute amount of the tested substance, a standard curve needs to be generated each time. Please follow the detailed protocol on the product datasheet to determine the concentration range for the standard curve.
posted April 2010
Protocol Id: 68
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