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HTScan^® FGF Receptor 3 Kinase Assay Kit

HTScan^® FGF Receptor 3 Kinase Assay Kit #7734

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Product Information

Product Description

The kit provides a means of performing kinase activity assays with recombinant human FGFR-3 kinase. It includes active FGFR-3 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula

Peptide substrate, Biotin-FLT3 (Tyr589): 1,945Daltons. GST-FGFR-3 Kinase: 82 kDa.

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5).

Alternate Names

enzyme; FGF; FGF-R3; FGFR; FGFR-3; FGFR3; high throughput; HTS; kinase assay; Tyrosine Kinase

Database Links

UniProt ID: P22607

Entrez-Gene Id: 2261

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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HTScan® FGF Receptor 3 Kinase Assay Kit #7734