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  3. HTScan® Src Kinase Assay Kit

HTScan® Src Kinase Assay Kit #7776

Inquiry Info. # 7776

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    Product Information

    Product Description

    The kit provides a means of performing kinase activity assays with recombinant human Src kinase. It includes active Src kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.
    Molecular Formula Peptide substrate, Biotin-peptide: 1830 Daltons. GST-Src Kinase: 90 kDa.

    Background

    The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

    Alternate Names

    c-Src; high throughput; HTS; htscankit; kinase assay; screening; Src; tyrosine kinase

    Database Links

    UniProt ID: P12931


    Entrez-Gene Id: 6714


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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