Render Target: STATIC
Render Timestamp: 2024-10-11T11:00:14.228Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:23:17.800
Product last modified at: 2024-06-27T13:37:04.390Z
1% for the planet logo
PDP - Template Name: Assay Kit Storage Temp
PDP - Template ID: *******69bdcf2

Human Anti-CD3/CD28 T Cell Activation Kit #70976

    Product Information

    Storage

    All components in this kit are stable for 12 months when stored at the recommended temperature.

    Protocol

    Product Description

    The Human Anti-CD3/CD28 T Cell Activation Kit provides the necessary antibodies to induce human T cell activation and proliferation using the soluble stimulation protocol.

    Specificity / Sensitivity

    The Human Anti-CD3/CD28 T Cell Activation Kit is used for the stimulation of human T cells following the soluble stimulation protocol.

    Background

    T cells are activated by signaling through ITAM (immunoreceptor tyrosine-based activation motif)-containing CD3 signaling chains that associate with the T cell receptor (1). Co-stimulation through CD28 on T cells provides an additional signal required for effective T cell activation (2). Signaling through both CD3 and CD28 can be initiated using the agonist antibodies Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) and Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free), respectively. If the agonist antibodies are used in the soluble stimulation protocol, cross-linking of their Fc domains using Goat Anti-Mouse Kappa Light Chain, F(ab')2 Antibody (Low Endotoxin, Azide-free) is required for optimal stimulation.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.