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35302
Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry)
Cellular Assay Kits
Assay Kit

Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry) #35302

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To Purchase # 35302S
Product # Size Price
35302S
1 Kit  (100 tests) $ 610

Product Includes Quantity (with Count)
Bafilomycin A1 54645 1 x 33 µg
SA-β-Gal Fluorescent Substrate 1 x 2 mg

Product Description

The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry) allows the quantitative measure of senescence-associated β-galactosidase activity in living cells via flow cytometry using a cell permeable fluorogenic substrate, which becomes fluorescent upon hydrolysis by β-galactosidase. The fluorescent molecule is optimally excited at 490 nm (blue laser) and peak emission occurs at 514 nm (usually FL1). Each kit contains sufficient quantities of reagents to perform up to 100 assays.

Background

Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent β-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
  1. Goldstein, S. (1990) Science 249, 1129-33.
  2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci., USA 85, 9086-90.
  3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-7.
  4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
  5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-51.

Pathways & Proteins

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Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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