|Product Includes||Quantity (with Count)|
|Senescence 1X Cell Lysis Buffer||1 x 25 ml|
|Senescence 2X Reaction Buffer||1 x 6.5 ml|
|Senescence Stop Solution||1 x 21 ml|
|SA-β-Gal Substrate||1 x 4 mg|
Additional Reagents (Not Supplied)
|1X PBS Wash||100 µL||400 µL||1000 µL||1500 µL|
|1X Cell Lysis Buffer||100 µL||400 µL||1000 µL||1500 µL|
NOTE: Cell treatments to induce senescence should be completed before initiating assay with this kit. Ensure that an untreated control is included, to provide a baseline measurement of fluorescence for comparison.
2X Assay Buffer Preparation: Immediately before use, add BME to 2X Senescence Reaction Buffer at a final concentration of 10 mM. Then dilute the 20X SA-β-Gal Substrate to 1X with 2X Senescence Reaction Buffer containing 10mM BME. Make only enough solution for the number of wells analyzed (50 µL per well). Use the 2X Assay Buffer immediately and properly discard any unused solution.
posted August 2020
Protocol Id: 2285
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