Use as directed in the CUT&RUN Assay Kit #86652 protocol. In detail, we suggest adding 3 μl 10% SDS Solution and an appropriate amount of Proteinase K (20 mg/ml) #10012 to each 300 μl CUT&RUN reaction to reverse formaldehyde crosslinks. This product can also be used in other assays at required amounts. Please make sure the 10% SDS Solution is completely in solution before use. If the product is not completely in solution, warm it up to ~50°C.
Store at room temperature. This product is stable for 12 months upon receipt when stored properly. If stored at lower temperatures, SDS may precipitate. Bring material to room temperature to make sure it is completely in solution before use.
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