|Product Includes||Volume (with Count)||Storage Temp|
|DNA Binding Buffer||2 x 30 ml||RT|
|DNA Wash Buffer (add 4x volume ethanol before use)||1 x 6 ml||RT|
|DNA Elution Buffer||2 x 1 ml||RT|
|DNA Purification Columns and Collection Tubes||1 x 36 Pack||RT|
Use as directed in the respective SimpleChIP® Chromatin IP kit and the CUT&RUN Assay kit protocols. Please note that if the following the SimpleChIP® Chromotin protocol there will be excess DNA Binding Buffer (#10007) to accommodate the CUT&RUN protocol.
Store at room temperature. Components are stable for 12 months upon receipt when stored properly.
This product is offered to enable DNA purification using spin columns with our CUT&RUN Assay Kit (#86652).
This product also is offered to conveniently provide additional ChIP buffer reagents for preparing, immunoprecipitating, washing, and eluting chromatin using our SimpleChIP® (#9002, #9003) and SimpleChIP® Plus (#9004, #9005) Enzymatic Chromatin IP Kits, as well as our SimpleChIP® Sonication Chromatin IP kit (#56383). These SimpleChIP® kits provide all the reagents required for performing the recommended of chromatin preparations (or optimizations) and chromatin immunoprecipitation (ChIP) assays, however there are instances where extra ChIP Buffer, ChIP Elution buffer, and NaCl are desired.
The DNA purification columns combine the convenience of spin-column technology with the selective binding properties of a uniquely designed silica membrane that allows for efficient recovery of DNA and removal of protein contaminants without the need for phenol/chloroform extractions and ethanol precipitations. After DNA purification, the enrichment of particular DNA sequences can be analyzed by a variety of methods.
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