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Render Timestamp: 2024-12-13T11:28:03.516Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-04-05 20:32:58.148
Product last modified at: 2024-12-05T18:15:08.262Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human BMP-2 Recombinant Protein #4697

    Product Information

    Product Usage Information

    ¶The working concentration of recombinant human BMP-2 generally ranges from 20-50 ng/ml.

    Storage

    Lyophilized product is very stable at -20°C. It is recommended to reconstitute with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 26
    Purity >95%
    Activity The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml.

    Source / Purification

    Recombinant human BMP-2 was expressed in E. coli and is supplied in a lyophilized form.

    Background

    Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs are synthesized as precursor proteins that are processed by cleavage to produce mature proteins. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting in the translocation of Smad to the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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