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Human CSF-1/M-CSF Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human CSF-1/M-CSF Recombinant Protein #8929

Citations (1)
The proliferation of M-NFS-60 cells treated with increasing concentrations of hCSF-1/M-CSF was assessed. After 48 hour treatment with hCSF-1/M-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
The purity of recombinant hCSF-1/M-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCSF-1/M-CSF and staining overnight with Coomassie Blue.
Western blot analysis of extracts from M-NFS-60 cells, untreated or treated with hCSF-1/M-CSF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

Product Usage Information


With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final hCSF-1/M-CSF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.

Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of hCSF-1/M-CSF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hCSF-1/M-CSF should be greater than 50 μg/ml.


Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 16-26
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCSF-1/M-CSF. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hCSF-1/M-CSF.
Activity The bioactivity of recombinant hCSF-1M/CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 0.5-15.0 ng/ml.
Molecular Formula Recombinant hCSF-1/M-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,403. DTT-reduced protein migrates as a 16-26 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 34-40 kDa protein. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino-terminal EEVSE of recombinant hCSF-1/M-CSF was verified by amino acid sequencing.

Source / Purification

Recombinant human CSF-1/M-CSF Glu33-Ser190 (Accession #P09603-3) was expressed in human 293 cells at Cell Signaling Technology.


Colony stimulating factor 1 (CSF-1)/macrophage colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). CSF-1/M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). CSF-1/M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. CSF-1/M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). CSF-1/M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in CSF-1/M-CSF expression may contribute to cancer progression, and high plasma CSF-1/M-CSF levels are associated with rheumatoid arthritis (1,4,5).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 8929
Cat. # Size Qty. Price
5 µg  (Carrier Free)