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Product last modified at: 2025-03-13T08:00:11.767Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human Erythropoietin Recombinant Protein #88073

    Product Information

    Storage

    Human Erythropoietin Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 18.4
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than 1 EU / 1 μg hEPO.
    Activity The bioactivity of recombinant human Erythropoietin (hEPO) was determined in a human TF-1 cell proliferation assay. The ED50 of each lot is 1.3-1.5 ng/mL.

    Source / Purification

    Recombinant hEPO was expressed in 293 cells and is supplied in a lyophilized form.

    Background

    Erythropoietin (EPO) is a 34 kDa protein that is required for the survival and terminal differentiation of erythrocytes (1). EPO is produced by several cell types, including tubular endothelial cells, interstitial cells, hepatocytes, Kupffer cells, astrocytes, and Schwann cells (2-4). EPO inhibits apoptosis and may protect neuronal cells from death during ischemia and/or neurodegenerative diseases (3,4). EPO inhibits pro-inflammatory cytokine production by inhibiting NF-κB signaling (5). In an animal model, EPO protected against the development of diabetes by promoting pancreatic β cell survival and growth (6). Binding of EPO to its cognate receptor, EPOR, induces activation of Jak2, Stat5, and Akt (3-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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